Abstract
Summary
In vitro propagation of Friend virus in mouse embryo tissue cultures has been observed by immunofluorescence and hemadsorption. Hemadsorption of chick erythrocytes was demonstrated. Parallel studies with immunofluorescence showed that the appearance of hemadsorption coincided with a build-up of viral antigen at the cell surface and in the cytoplasm. Even though no gross changes were observed in the infected cells, a virus-induced modification of the Friend virus infected cell surface was indicated. Hemadsorption can now be used for quantitation of the virus in culture, thus circumventing the cumbersome bioassay in mice.
Get full access to this article
View all access options for this article.