Explanted Nasal Mucosa

Abstract

Summary

Explants derived from the mucosa of benign nasal polyps were cultured in closed vessels by a coverslip technic. The polyps had previously been resected from children with cystic fibrosis of the pancreas and a control. The explants grew as fenestrated, multilayered membranes with an appearance suggesting primitive aggregation. The pattern occurred again when the original cell sheets were disaggregated and allowed to grow. Perhaps the mucosal layer was the origin of some of the cells. Use of electron microscopy and cytochemistry was not helpful in differentiating possible cell types.