Virus-Free Infective Centers Produced by Photodynamic Inactivation of Poliovirus in Rhesus Kidney Cell Suspensions

Abstract

Summary

When suspensions of rhesus monkey kidney cells (LLCMK2) are inoculated with proflavine-photosensitized poliovirus, type 1, incubated 20 minutes at 36°C in the dark, and then irradiated with bright visible light, all detectable virus in the suspension is destroyed, but 0.01 to 0.1% of the cells are capable of developing into infective centers. This finding suggests that RNA from proflavine-photosensitized poliovirus loses its photosensitivity in the early stages of cellular infection, or, alternatively, becomes stabilized shortly after penetration of the cell.