Incorporation of Tritium from Succinate-2,3-H. 3 into Long Chain Fatty Acids by Aortic Mitochondria. ∗

Abstract

Summary

The mechanism of succinate stimulation of fatty acid synthesis by aortic mitochondria was studied. Tritium, but not carbon, from succinate labeled in positions 2- and 3- is incorporated into long-chain fatty acids in this system. The relative incorporation of tritium in various fatty acids is similar to that of C¹⁴ from acetate. Succinate can, therefore, provide protons for the reductive steps. We believe this is a demonstration of energy-requiring reversal of electron flow participating in a synthetic process and another way in which Krebs Cycle acids can influence fatty acid synthesis.