Abstract
Summary
A method for determination of free fatty acids in plasma is presented, together with tests for the validity of the procedures used. Total lipids are treated with excess ethereal C14-diazomethane, and radioactivity due to C14-methyl esters of fatty acids is isolated by silicic acid column chromatography, and counted. Similar treatment of known amounts of standard fatty acid yields a curve from which the plasma free fatty acid concentration may be obtained. Use of 0.5 ml of plasma per determination gives optimal results, but the method may be employed with 0.1 ml plasma or less.
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