Abstract
Summary
Plaques were produced by mouse-adapted strains of the 6 recognized Dengue serotypes using a modified overlay of cultures of rhesus monkey kidney cells. Overlay modifications included changes in the magnesium and calcium ion concentration and addition of Larginine and oxalacetic acid. Plaques 1-3 mm in diameter appeared 5-11 days after inoculation of cultures. Endpoints were generally higher in rhesus kidney cultures during parallel titrations of the virus in tissue culture and mice.
Get full access to this article
View all access options for this article.