Abstract
Summary
Five different tissue culture media prepared as a dry powder by blending the ingredients in a ball mill followed by desiccation were reconstituted and evaluated for their ability to support cell growth of ten different tissue culture cell lines. Eagle's BME, Eagle's MEM, Medium 199, NCTC 127 and NCTC 109 supported good cell multiplication as compared to the control Eagle's MEM prepared from 10 × or 100X concentrated liquid stock solutions. Plating efficiencies of 8 of 10 cell lines were higher in media prepared from the powdered Eagle's MEM than in the control media, Plating efficiency data were confirmed by growth curves in roller tubes and by growth of massive inocula in milk dilution bottles. Additional important advantages of using dry powdered culture media include: low cost of powdered media as compared to the present commercial liquid concentrates; greater stability; greater uniformity of the chemical ingredients because of the ball milling process and the rigid selection of large bulk lots of basic ingredients; low shipping charges; and ease of storage for long term experiments.
The authors wish to thank Drs. Richard C. Meyer and Robert E. Stevenson, Nat. Inst. Health, and Herbert R. Morgan, Univ. of Rochester Med. School, N. Y., whose efforts as members of the Subcommittee on Media and Substrates of the Cell Culture Collection Committee initiated this study. Thanks are also due Dr. Leonard Hayflick, Wistar Inst., for helpful advice, and Mr. Michael Peluse and Mrs. Elizabeth Kauderer for technical assistance.
Get full access to this article
View all access options for this article.