Abstract
Summary
A method for uptake of a new hydrophobic fluorescent dye by human blood platelets is described in which cell concentrations are constant and those of dye varied widely. Uptake is measured in isotonic saline buffered with EDTA at a pH of 3.5, where cells are near their isoelectric point and dye maximally affixed to them. Dye uptake did not fit usual patterns of adsorption. At all platelet weights, the amount of added dye bound increased with increasing strengths of dye, being nearly proportional to the square of the concentration of remaining dye. Data from several normal platelet donors show maxima in per cent of dye taken up and slight differences in this per cent of dye over a wide range of dye added. This suggests varying amount of hydrophobic substances in or on platelets and a means for their assay.
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