Infectious Ribonucleic Acid (RNA) of Japanese B Encephalitis (JBE) Virus

Abstract

Summary

H₂O, 1.0 M Na₂SO₄, and 1.5 M MgSO₄ protected the JBE virus from inactivation at 50°C, whereas 0.1 M and 1.0 M NaCI, 0.1 M Na₂SO₄ as well as 0.15 M MgSO₄ inactivated the virus. However, the JBE-RNA was more stable in the presence of a high concentration of NaCl than in H₂O and other salts tested. In addition, when EDTA was used either as a solution for homogenization of infected mouse brains or as a suspending solution of extracted infectious JBE-RNA, especially in the former case, the PFU of the RNA which were measured on chick embryo cell monolayers closely approached that of the virus and the infectivity of the RNA was protected from inactivation.