Plaque Studies with Certain Group B Arboviruses. II. Japanese B Encephalitis Virus on Hamster Liver Tissue Cultures. ∗

Abstract

Summary

1. Monolayer hamster liver tissue cultures proved to be highly susceptible to JBE virus on the basis of CPE titers. Primary HKTC, HLTC and mixed cultures were found to be equally sensitive for several virus strains. Secondary HLTC was less sensitive than the primary. 2. Nakayama and OCT-wild strains of JBE produced similar plaques on HLTC using the double agar overlay technique at 37°C, but the method was not as sensitive as CPE for virus detection. Plaques were 0.4-0.7 cm in diameter and clear with sharp boundaries. 3. Plaques were produced on secondary HLTC using the double agar overlay technique at 37°C . These were smaller, and hazy but in increased numbers. Mixed kidney and liver cultures failed to produce plaques.