Abstract
Summary
An assay method involving the use of radioactive biotin and Sephadex chromatography was used to determine avidin and/or the avidin-biotin complex. It was demonstrated that avidin is relatively heat labile while the avidin-biotin complex is relatively heat stable. It was found that the affinity of avidin for biotin, essentially zero in aqueous solution, is a direct function of the ionic strength of the medium in which avidin and biotin react.
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