Abstract
Summary
An improved anti-inflammatory assay based on the reversal of endotoxin-induced lung inflammation in mice is described. Changes in design of the original apparatus have: a) increased the number of animals which can be exposed to endotoxin; b) decreased the amount of endotoxin needed for maximum inflammatory response. A direct correlation exists between concentration of endotoxin and the resulting increase in weight of the lung and degree of histopatho-logical changes. The advantages of this method for testing anti-inflammatory activity of steroid as well as non-steroid compounds are discussed in comparison with other methods described in the literature.
We gratefully acknowledge the histopathological evaluations done by Mr. John A. Tornaben and Dr. John P. Manning, Dr. Robert L. Kroc is thanked for his support, interest and encouragement throughout this work.
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