Abstract
Summary
Succinic (SDH) and lactic (LDH) dehydrogenase activity was studied histochemically in the regenerating forelimb of adult Triturus. During the stages of wound healing and of blastemal cell accumulation, SDH activity decreased in the tissues underlying the wound epidermis while LDH increased. During stages of rapid growth of the regenerate, SDH was not histochemically demonstrable in the mesenchymatous tissue; but LDH activity was high. As the tissue differentiated in later stages of regeneration, SDH activity increased while LDH in general declined. The wound epithelium showed both LDH and SDH activity throughout regeneration with variations in intensity less striking than in underlying parts. The results of our work and of others indicate that in the stages of appearance, accumulation and proliferation of the blastema, metabolism via the Krebs cycle is replaced by glycolytic and/or the pentose pathway metabolism.
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