Abstract
Summary
Intact hemidiaphragms from normal female rats (60-75 g) were utilized to study the effect of insulin on C14 L-proline transport as well as its incorporation into protein as a function of protein synthesis. Bovine insulin (0.1 unit/ml) significantly increased the intracellular accumulation of pro-line when protein synthesis was maximally reduced by addition of puromycin to the tissue. It is concluded that the stimulation of amino acid transport into muscle cells by insulin is independent of the increase in protein synthesis effected by the hormone.
We are indebted to Dr. M. A. Root, Eli Lilly & Co., for the gift of insulin.
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