Abstract
Summary
Serially transferred hamster or human fibroblasts maintained on synthetic medium containing 10% calf serum could be carried through about 10 and about 50-70 cell generations respectively, before growth ceased. Addition of crystallized serum albumin to the medium to a concentration of 20 mg/ ml affected the growth potential so that in both cases over 100 cell generations could be obtained. The human fibroblasts after 95 cell generations showed no chromosomal abnormalities while in the hamster fibroblasts some deviation from the diploid number did occur. In neither case was there evidence of the evolution of improved growth properties that characterize established cell lines.
Get full access to this article
View all access options for this article.