Abstract
Summary
Rat, sheep, and dog liver homogenates and microsomal preparations were assayed for glucuronosyl transferase activity and ability to regenerate UDPGA from glucose. Sheep liver displayed a greater capacity to conjugate H4E, p-nitrophenol, and phenol-phthalein than did similar preparations from dogs and rats. This enhanced ability was attributed to an increase of glucuronosyl transferase activity and the ability to regenerate UDPGA from glucose. In addition, glucose was observed to be a better precursor for regeneration of UDPGA than galactose with sheep homogenate preparations. Dog liver was found to conjugate H4E poorly, but was able to conjugate p-nitrophenol and phenol-phthalein.
The authors would like to thank Dr. John J. Schneider for his generous gift of H4E-glucuronide which was used as a reference standard in the electrophoretic studies.
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