Abstract
Summary
These studies have shown that the HeLa cell strain in fluid media is a satisfactory host cell system for arbovirus titrations as well as for neutralization tests. Of 94 arbovirus strains tested, 89 grouped as well as ungrouped viruses caused constant and regularly occurring destructive changes in HeLa cells. Partial destruction of the cell sheet was observed with one-third of the viruses tested, subtotal necrosis with another third and total necrosis with the remainder. In one instance, the cytopathic effect progressed only to a stage characterized by presence of multiple focal areas of necrosis. In 5 instances, no CPE was obtained. As far as tested, specific antiserum prevented development of CPE. Fifty-four strains were propagated serially without difficulty.
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