Induction of Neoplasms in Salivary Gland Rudiments Recovered from Frozen Storage

Abstract

Summary

Viability of submandibular salivary gland rudiments of mice was demonstrated after slow-freezing and storing in a 5% glycerol medium at — 150°C. Development of the rudiments after thawing was less rapid and less extensive than with fresh rudiments, but the capacity of the organ to develop morphologically characteristic neoplasms in response to polyoma virus was not appreciably impaired.