Electrophoretic Behavior of Bile Acids and Cholesterol in Gall Bladder and Hepatic Bile. ∗

Abstract

Summary

1. Human gall bladder and hepatic bile, obtained after oral administration of cholic and chenodeoxycholic acid-24-C¹⁴, were analyzed by paper electrophore-sis. Similar studies were performed with bile supplemented in vitro with cholesterol C¹⁴. 2. In gall bladder bile the main part of the labeled cholesterol and the bile acids migrated faster than albumin. 3. In hepatic bile the bile acids migrated at a rate similar to that of albumin whereas most of the cholesterol remained at the point of application. 4. The differences in electrophoretic mobilities of cholesterol and bile acids in hepatic bile and gall bladder bile respectively can be explained by differences in concentration of the bile constituents. 5. Cholesterol in gall bladder bile is in solution as a complex with a high electrophoretic mobility. This complex is not a lipoprotein and easily dissociates by dilution of the bile into a complex with no electrophoretic mobility. The bile acids are of importance for the formation and stabilization of the high mobility complex.