Failure of Ethanol to Stimulate Incorporation of Acetate-1-C 14 into Hepatic Fatty Acids in vitro ∗

Abstract

Summary

Experiments conducted to determine the effects of ethanol on fatty acid synthesis in liver slices indicate that ethanol, at all concentrations tested, suppresses the incorporation of labeled carbon derived from acetate-1-C¹⁴ into hepatic fatty acids in vitro. This suppression is explained as being mostly due to isotopic dilution of labeled acetyl-CoA derived from acetate-1-C¹⁴ by unlabeled ace-tyl-CoA derived from ethanol, although the inhibitory effect of ethanol on the metabolism of acetate in liver slices may also be partly responsible for this suppression.