Use of Dielectric Heating (Shortwave Diathermy) in Thawing Frozen Suspensions of Tissue Culture Cells. ∗

Abstract

Summary

1. Thawing of frozen mammalian cells by well controlled shortwave diathermy is feasible. 2. Such thawing causes no apparent injury to the cells. 3. Shortwave diathermy offers no advantage over agitation in a 37°C water bath for thawing small masses of frozen tissues and cells. 4. The use of shortwave diathermy appears to be desirable for thawing large masses of tissue such as the dog or human kidney and the present studies indicate that this method can be safely used.