Abstract
Summary
A cold perchloric acid extract was made of the skins of young rabbits, and treated with Norit to provide a concentrate which was then fractionated on Dowex-1 chloride. All fractions were investigated for the presence of peptide-nucleotide components by absorption spectrophotometry, nin-hydrin analysis, paper electrophoresis and treatment with hydroxylamine. Small amounts of substances reacting with alkaline hydroxylamine were detected in preparations prior to column chromatography; eluted fractions, however, did not yield positive hydroxamate tests in the test tube. Two of the fractions when subjected to paper electrophoresis contained overlapping nucleotide and peptide components migrating toward the cathode, and indeed yielded positive color tests for hydroxamates after treatment with hydroxylamine and ferric perchlorate spray reagents. Proline was present in these fractions as well as in several others. While hydroxyproline was present in the Norit eluate, it was not retained on the Dowex-1 column and therefore did not appear in any of the eluted fractions either in free or combined forms. An acidic peptide containing glutamic acid (as N-terminal), glycine and cysteic acid was isolated from an eluted fraction which also contained adenylic acid.
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