Abstract
Summary
Lipids from connective tissue cells cultivated in vitro were identified by silicic acid column and paper chromatography, and by Dawson's hydrolysis procedures. Phospholipids constituted 64–84% of the total lipid with the remainder approximately equally distributed between cholesterol and the glycerides. Total lipid concentration ranged from 9.1 to 17.2 mg per 100 mg of dry cell solids. Phosphatidyl choline,-ethanol-amine,-myoinositol, sphingomyelin, and plasmalogens were identified in all cells examined. Phosphatidyl serine was undetected in one, and “phosphatidic acid” undetected in several of the cell lines. No unusual intracellular concentration of lipid or definite correlation with mucopolysaccharide production was demonstrated.
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