Insulin Secretion from the Isolated Pancreas in Absence of Insulinogenesis

Abstract

Summary

The effect of dinitrophenol (DNP) on glucose stimulation of the release of immunologically measurable insulin was studied in the isolated perfused pancreas of the rat. In a 3-hour perfusion at 5 × 10⁻⁴ m DNP, protein synthesis, as measured by incorporation of valine-C¹⁴ into total pancreatic protein, was completely inhibited. The release of insulin by glucose, however, was unimpaired. Results indicate that glucose (or a product resulting from glucose metabolism) can stimulate insulin secretion directly and that a prior stimulation of insulinogenesis from amino acids is not required.