Abstract
Summary
Methods for prolonged storage at low temperatures and recovery of antibody-producing cells from spleens of preimmunized mice were investigated. A method was devised which permits the cells to be stored with no decline in their capacity to synthesize antibodies upon subsequent in vivo culture. The method consists of suspending cells in 10% dimethyl sulfoxide, decreasing the temperature of the suspension gradually to −50°C followed by storage at −196°C. The extent to which antibody-producing cells are damaged by certain other freezing procedures was evaluated.
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