Abstract
Summary
Anti-DNP, anti-pipsyl and anti-ovalbumin antibodies have been isolated from the peritoneal fluids of individual hy-perimmunized mice. On immunoelectrophoresis, the antibody preparations appeared to contain, regardless of specificity, at least 2 families of molecules with slightly different electrophoretic mobility, possessing similar as well as distinct antigenic determinants. After reduction and alkylation in 8M urea and starch gel electrophoresis in 8M urea, the anti-hapten antibodies yielded distinct bands corresponding to L chains, in contrast normal mouse 7S γ-globulin and purified anti-oval-bumin antibodies showed smears corresponding to the zone of L chain migration.
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