pH,Protein,and Semliki Forest Virus Infectivity. ∗

Abstract

Summary

SFV retained a high level of infectivity from pH 6 to above pH 10 when serially diluted in 0.1 m phosphate buffer. After incubation of such serial dilutions at 37°C, infectivity persisted at 10^-3 dilutions only above pH 6. The presence of 0.1% bovine serum albumin resulted in a marked increase in thermal stability at 37°C above pH 6 but not in more acid solutions. On dilution in several tissue culture media both SFV and WEE retained a higher level of infectivity in media containing a mixture of amino acids or glutamine than in media containing glucose and inorganic salts only.