Connective Tissue VIII. Factors Effecting Collagen Synthesis by Sponge Biopsy Connective Tissue. ∗

Abstract

Summary

The conversion of C¹⁴-proline to C¹⁴-hydroxyproline has been used as a measure of the synthesis of collagen in sponge biopsy connective tissue in vitro. Krebs-Henseleit medium, pH 7.4, under an atmosphere of 95% O₂–5% CO₂ created the most satisfactory conditions we have employed for collagen synthesis in vitro. Elimination of NaCl from the incubation medium gave the most striking depression of collagen synthesis. Replacement of NaCl by other chemical compounds did not restore the synthesis of collagen to the control level. An amino acid mixture, containing essential amino acids, had an inhibitory effect on collagen synthesis. Freezing and crushing destroys most of the enzyme system or systems required for collagen synthesis. The data presented are compatible with the concept that all collagen hydroxyproline is derived from proline peptide.