Abstract
Summary
Infection of FL tissue culture cells with M. gallisepticum, M. agalactiae and M. hominis was achieved, while the saprophytic PPLO M. laidlawii A could not be detected in such cells when cultivated for intervals longer than 2 days following infection. Parasitic PPLO remained in cell cultures for periods up to 3 weeks. After prolonged incubation, cytopathogenic effect could be demonstrated in cultures infected with M. gallisepticum. In addition, tissue cultures infected with M. gallisepticum showed an accumulation of acetate in growth fluids. The latter observation, supported by appropriate isotope data, strongly suggests that acetate appears as a product of lactate dissimilation by this PPLO.
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