Effect of Deuterium Oxide on Lipid Components of L-5178Y Cells. ∗

Abstract

Summary

A comparison has been made of the relative lipid content of L-5178Y cells grown in conventional medium or in the same medium containing 30% D₂O. Cells grown in D₂O medium contain more total lipid. The major increases in the lipids of the D₂O grown cells are due to increases in the levels of triglycerides and sterol esters. There are very few differences in cellular levels of any of the other lipid fractions. Preliminary isotope experiments indicate that the increased lipid content in the D₂O grown cells is due to increased synthesis of lipid.