Abstract
Summary
Folate activity was determined in the whole blood and tissues of 70 normal rats, before and after loading with PGA using 3 different bio-assays simultaneously. L. casei was used to measure “total folate activity,” S. faecalis to estimate monoglutamate activity as well as that of the reduced forms of PGA, and P. cerevisiae to determine only the reduced folate activity.
L. casei activity before loading with PGA was about 25 times higher in the peripheral blood than when measured with S. faecalis and P. cerevisiae. On the other hand, the ratio of activity with L. casei, S. faecalis and P. cerevisiae in liver, kidney, spleen and intestines was only 1.4:1:1. It was concluded that the reduced forms constitute over 50% of the folate derivatives in rat tissues.
Intraperitoneal injection of 2 μ/g PGA was followed by a temporary elevation of the activity with the L. casei and S. faecalis assays which disappeared within 6–24 hr after the injection. No significant increase was noted in the P. cerevisiae assay following PGA load.
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