Abstract
Summary
An attenuated strain of poliomyelitis virus (CHAT) will not grow in monkey kidney cells at 40°C. When deuterium oxide (25–40%) is present in the medium, replication of CHAT virus will take place at 40°C. Since both deuterium oxide treatment and irradiation with X-rays yield “giant” cells, the 2 treatments have been compared for their ability to support the growth of CHAT poliovirus at 40°C. At several levels of X-irradiation, monkey kidney cells will not support the growth of CHAT virus at 40°C. When D2O is added to the medium of the X-irradiated cells at 40°C, replication of CHAT virus is observed. The effect is not due to cell size or number.
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