Influence of 2 α -Methyl-17 β -Hydroxy-5 α -Androstan-3-one on Incorporation of Glycine-2-C 14 into Proteins of a Rat Mammary Fibroadenoma. A Possible Bioassay Method. ∗

Abstract

Summary

The uptake of glycine-2-C¹⁴ into the proteins of a rat mammary fibroadenoma has been studied as a possible bio-assay procedure for detecting useful anti-tumor steroids and other substances for further studies in humans. A preliminary method has been evolved consisting in transplantation of the tumor to 140 ± 15 g female rats and after 35 days injecting the rats once daily for 14 days with the test compounds. On the last day of treatment the rats received one injection of glycine-2-C¹⁴. Twenty-four hours after the last injection of the test compound and the glycine-2-C¹⁴ injection, the tumors are removed, weighed and C¹⁴ specific activity of the tumor protein determined. The tumor was inhibited by testosterone propionate but more regularly and more intensely by equal weights of 2α-methyl-17β-hydroxy-5α-androstan-3-one, a steroid.