Use of Centrifugal Force to Promote Adsorption of Myxoma Virus to Cell Monolayers. ∗

Abstract

Summary

Centrifugation of myxoma virus onto preformed monolayers of RK and RHF-1 cells substantially increased the number of PFU compared to other methods of virus adsorption. The maximum number of PFU was obtained after only 15 minutes of centrifugation at 1900 g at room temperature. Virtually simultaneous infection of all RK cells growing as a monolayer on the bottom of shell vials can be accomplished by centrifuging a low multiplicity of myxoma virus onto the cells at 1270 g for 10–20 minutes.