Ascites Tumor Fluid as a Source of Antibodies in Mice

Abstract

Summary

Ehrlich ascites and Gardner lymphosarcoma tumor cells were employed to induce ascites fluid formation in mice. Two strains of mice injected with antigens (X_p-human γ-globulin, myosin, ovalbumin) mixed in Freund's adjuvant had the capacity to respond with antibody production. The antibodies developed to X_p-human γ-globulin reacted with the hapten portion as well as normal human γ-globulin. The mouse anti-human myosin serum behaved similarly to the rabbit anti-myosin serum when studied by the immunohistochemical method.