Abstract
Summary
A rapid and simple method is described for preparing specimens of an intra-erythrocytic parasite for morphological examination in the electron microscope. The technic includes dehemoglobinization of the erythrocyte by dialysis against a hypotonic solution of a volatile salt. The hemolyzed stromata are transferred to specimen grids by gravity sedimentation. Three principal artifacts associated with the method of preparation are noted. The complex structure of A. marginale as reported by previous workers is confirmed and extended by these results. A possible relationship between certain observed forms and the parasite is suggested.
While the National Animal Disease Laboratory was located at Beltsville, Md., blood samples for this work were supplied by Drs. D. W. Gates and T. O. Roby and Mr. James Caudill, of the Beltsville Parasitological Lab., Animal Disease and Parasite Research Division, Agri. Research Service, U. S. Dept. of Agriculture. The author gratefully acknowledges their assistance.
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