Abstract
Summary and conclusions
Measurements of relative hemolytic activity of varying mixtures of complement, red cells, and hemolysin with added heterologous protein reveal inhibition or apparent partial complement fixation in the absence of known previous immunization. The added protein in these studies is either hemoglobin or human gamma globulin. This inhibition is absent when the hemoglobin and complement are derived from the same specimen of blood. These data reveal an important possible source of error when quantitative estimates of antibody formation are made using complement fixation tests.
I would like to acknowledge gratitude to Drs. A. E. Lewis and G. R. Biskind for invaluable suggestions during the development of this project and for extensive aid in preparation of this manuscript.
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