Abstract
Summary
Polysaccharide isolated from encapsulated Klebsiella pneumoniae, type B, has been found to consist of glucose, glucuronic acid and rhamnose. C14 was incorporated biosynthetically into these 3 components from acetate- 1-C14 added to the media. Following injection of the C14-labeled polysaccharide into animals, up to 80% of the labeled material has been recovered from animal tissue by a combination of enzymatic and extractive procedures. Much of the C14-la-beled material in tissue retained its original haptenic properties and relative C14 content of the constituent monosaccharides.
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