Growth of L-M Strain Mouse Cells in a Chemically Defined Medium. ∗

Abstract

Summary

Using the criteria of generation time, final population density, and chromosome analysis, it has been demonstrated that Eagle basal medium is adequate for continued propagation of L-M strain mouse cells both as monolayers and in suspension. Three distinct advantages to this system are: (1) the relative simplicity of the medium in terms of number of components as compared to other defined media currently available, (2) the 3- to 5-fold increase in cell population achieved in 7 to 10 days, in contrast with results using most defined media, (3) the growth of these cells in suspension culture, which is an ideal system for both nutritional and parasite-host cell relationship studies. The effectiveness of methylcellulose as a physical protective agent for cells grown in a simple, chemically defined medium has been demonstrated.