Liver Ketogenesis V. In vitro Acetoacetate Production and Utilization in Rats Fed High Fat-high Casein Diet. ∗

Abstract

Summary

The results in general indicate that the antecedent diet leaves its imprint on the metabolism of isolated tissues, causing profound changes in the metabolic pattern, depending on the type of such diet. The gain in body weight of rats fed high casein and high fat-high casein alone or in combination with thyroid, is found to be less than that in normals. These rats were observed to have in varying degrees hyperglycemia, fatty liver and hypertrophy of liver, kidney and adrenal tissue. Feeding of high fat-high case-in diet alone, or with thyroid, induces overproduction of acetoacetate, both endogenous and exogenous, by liver slices. Utilization of acetoacetate by the kidney slices obtained from such rats is not, however, increased. Consumption of oxygen by liver and kidney slices of rats fed high casein and high fat-high casein is less than that in normal tissues. The metabolic disturbances observed in the tissues of rats fed diets low in carbohydrate but rich in protein or fat plus protein, are aggravated by prolonged feeding of the same diet (10 weeks as compared to 4 weeks).