Abstract
Starvation for periods up to twelve days does not reduce the amino-acid content of the tissues of dogs, nor does high protein feeding (500 grams of beef daily added to the regular diet for 1 to 7 days) increase it. The results indicate that:
1. Nitrogen retained as the result of high protein feeding is not in the form of stored digestion products, but rather as body protein.
2. The free amino-acids of the tissues can originate not only from the food, but also from autolysing tissues, as the latter are the only apparent source from which the amino-acid supply can be maintained during starvation.
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