Abstract
Summary
The uptake of triiodothyronine-I131 by bull spermatozoa was studied. Trials with frozen semen (-79°C) and stored semen (5°C) indicated that uptake of T3-I131 by spermatozoa was related to viability of the spermatozoa. Uptake of T3-I131 by spermatozoa was independent of the amount of T3 added to the diluted semen within the range studied. The composition of the spermatozoa diluent greatly influenced uptake of T3-I131 by spermatozoa. Washed spermatozoa suspended in a 3% sodium citrate diluent had a much greater uptake of T3-I131 than washed spermatzoa suspended in an egg yolk-sodium citrate diluent, indicating that diluent constituents may compete with the spermatozoa for uptake or binding of T3-I131.
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