Histochemistry LXVII. Microscopic Microbiological Assay. Determination of Biotin to 10 -15 Gram. ∗

Abstract

Summary

A technic for microscopic microbiological assay was elaborated in which droplets of total incubation mixture down to 0.010 μl volume were employed under oil. Measurement of bacterial growth by light scatter was used. The technic was applied to determination of biotin to 10¹⁵g with approximately the same percent accuracy and precision as the macrotechnic with ml-volumes.