Abstract
Summary
Normal primary bovine kidney cultures, partially depleted of endogenous nutrients, utilized pyruvate at a 2 1/2- to 4-fold greater rate than glucose. The pathways of pyruvate metabolism were not clearly defined. Pyruvate was not appreciably metabolized oxidatively since oxygen uptake could account for only 6-29% of the total pyruvate consumed and malonate only slightly decreased its utilization. Pyruvate, singly, did not form lactate and was used to a greater extent as glucose concentration decreased. Glucose utilization, close to maximal at 1 × 10-2 M and dependent on glucose concentration, was independent of pyruvate concentration. Virus infection did not change the rate of pyruvate utilization in contrast to the increase found in glucose uptake. Lactalbumin hydrolyzate did not yield lactic acid of itself but did stimulate glucose uptake and corresponding lactate formation. Acetate was not oxidized nor produced lactate.
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