Abstract
Summary
Kidney renin and angiotensinase concentrations were determined for the following groups of rats: normals(7); rats with renal hypertension produced by unilateral clipping of the renal artery (12); and rats unilaterally clipped which failed to develop hypertension(11). Kidney extracts were assayed for renin by 2 methods: 1) direct intravenous injection into a rat sensitized by ganglionic blockade, and 2) in vitro production of angiotensin which was subsequently assayed in a similar manner. Angiotensinase concentration was determined by incubation of the kidney extract for 2 hours at 37° with a known amount of synthetic angiotensin and subsequent assay of the residual angiotensin. Renin concentration was reduced in kidneys of both groups of rats with renal artery clips. The reduction in the untouched kidney was greater than that in the clipped kidney. Concentration of angiotensinase in the kidneys of experimental animals which became hypertensive was equal to that in control animals.
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