Abstract
Summary
Detailed studies were made of the chemical nature of carbon-14 activity found in acid-soluble nucleotide, ribonucleic acid, and deoxyribonucleic acid fractions isolated from kidney or liver tissue of rats rendered experimentally nephrotic with a series of 10 small daily injections of aminonucleoside-8-C14, and, in similar fractions obtained from rats sacrificed as early as 3 hours after administration of a single large dose of the labeled compound. No significant differences were noted. Only the adenosine and guanosine phosphates of the acid-soluble nucleotide fractions and the adenine and guanine bases of RNA and DNA hydrolysates were labeled with carbon-14. No evidence was obtained either for conversion of the intact aminonucleoside to the corresponding nucleotide or for incorporation of the 6-dimethylamino- or 6-methylamino-purine moieties into ribonucleic acid or deoxyribonucleic acid of kidney or liver tissue.
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