Abstract
Summary
A procedure has been described whereby species-associated antigens were directly identified in cultured mammalian cells by fluorescent antibody techniques. Guinea pig anti-cellular globulin fractions were conjugated with fluorescein isothiocyanate. Dispersed cells were prepared from monolayer cultures or frozen cell suspensions and the resulting wet cell suspensions were treated with the labeled antibodies. Staining specificity was recognized by presence or absence of peripheral fluorescence of the treated cells. Four cell strains derived from human tissues, 3 from mouse, and one from Chinese hamster, reacted specifically with homologous but not with heterologous labeled antibodies. Antibodies to monkey- and human-derived strains exhibited reciprocal cross-reactions that were eliminated by absorption procedures.
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