Abstract
Summary
Poliovirus was regularly recovered from the agar surface of bottle cultures exhibiting plaques. From the area immediately surrounding a plaque virus recovery was frequent. Only rarely was virus isolated at a distance therefrom. Virus was also often recovered from the glass walls. In cultures simultaneously inoculated with 2 different antigenic types of poliovirus and no material subsequently added, the agent isolated from a plaque consisted of a single antigenic type provided the bottles were maintained in the inverted position. When a solution of neutral red was added several days after inoculation of 2 antigenic types of virus, both types were recovered from a single plaque.
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