Abstract
Summary
Ingestion of polystyrene latex (PSL) particles measuring 0.264 μU in diameter has been demonstrated in chicken fibroblasts in vitro. PSL was added to cultures of chicken fibroblasts grown on carbon-collodion coated steel grids and these mounts removed at intervals of I/2 to 24 hours. The fixed and metal shadowed whole cell preparations revealed PSL in various stages of entry into these cells. PSL was found in invaginations of the cell wall and in vacuoles in the cytoplasm. Sections of cultured fibroblasts inoculated with PSL revealed PSL particles in cell vacuoles. The rate of particle uptake, however, was too low to be measured by the method used.
The author wishes to thank Doctors M. J. Moses and G. A. Meek for their interest in this work and their helpful suggestions.
Get full access to this article
View all access options for this article.