Abstract
Summary
With a perfused, isolated rabbit heart LPL activity appeared in the perfusion solution of bicarbonate buffer in the presence of 5 mg % heparin and 5 to 10% serum. However, under the same conditions, LPL did not appear with phosphate buffer. The LPL produced was inhibited by protamine sulfate, CaCl2 and NaCl. Optimum pH of enzymatic activity was at pH 8.2, and 80% of the activity was lost by heating at 50°C for 2 min and 70°C for 30 sec. After perfusion, LPL activity in the heart disappeared. Total activity appearing in the circulating solution was nearly equal to total activity of heart tissue which was extractable with ammonium buffer solution.
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